Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 50 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659758.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 50 G
HEPES, Free Acid is a critical biochemical reagent for maintaining pH stability in cell cultures and biochemical experiments. This 50g package is ideal for smaller scale applications, where precise pH control is essential for the success of experiments. HEPES is effective in a pH range of 6.8 to 8.2, which makes it a preferred choice for a variety of applications including enzymatic assays, electrophoresis, and cell-based assays. Due to its excellent buffering capacity and low toxicity to cells, HEPES ensures that pH variations are minimized during extended incubation periods, supporting reliable and reproducible experimental outcomes.
![Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid], sodium salt, 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660261.jpg-250.jpg)
Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid], sodium salt, 1 Kg
MES Sodium Salt (2-(N-Morpholino) ethanesulfonic acid), sodium salt, 1 kg, is a biochemical reagent used to maintain a stable pH in various laboratory applications. It is particularly effective in experiments involving cell culture, enzyme assays, and protein purification. The sodium salt version of MES provides enhanced ionic strength, making it suitable for applications requiring a more robust buffering system. This 1 kg volume is ideal for laboratories with ongoing experiments that require a steady supply of buffer for high-throughput assays or large-scale procedures. MES Sodium Salt ensures consistent pH levels without interfering with the biological systems being studied, making it an essential reagent for molecular biology and biochemistry.
![Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660136.jpg-250.jpg)
Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 250 G
MOPS (3-(N-morpholino)propane-1-sulfonic acid) is a biochemical reagent designed for maintaining pH stability in a variety of laboratory procedures. This 250g package of MOPS is ideal for preparing moderate quantities of buffer solutions used in research, especially in molecular biology, protein chemistry, and biochemistry. MOPS is commonly employed in processes that require precise pH control, such as protein purification, enzyme assays, and electrophoresis. It is particularly effective in the pH range of 6.5 to 7.9 and is widely used to stabilize nucleic acids, proteins, and other biological molecules in experimental conditions.
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 6.0, 500 mL
Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications.The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information:pKa (25 °C): 7.1 (Free acid)Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.Appearance: LiquidPH Value: 6.0
West Pharmaceutical Services Inc WEST PHARMACEUTICAL SERVICES INC
NC3936200 V-35 4432/50G SIL 1000/CS
Sigma Aldrich Fine Chemicals Biosciences MES sodium salt 99 titrati25G
MES sodium salt is commonly employed as buffer. Useful pH range for MES sodium salt is 5.5 to 6.7.
Dojindo Molecular Technologies Inc TAPSO (25G), Good's buffer, CAS: 68399-81-5, pka=7.7, Active pH 7.0-8.2, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
Dojindo Molecular Technologies Inc ADA (25G), Good's buffer, CAS: 26239-55-4, pka=6.6, Active pH 5.8-7.4, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
Promocell GmbH PromoCell Hepes BSS (30 mM) 30 ml, HEPES Buffered Saline Solution
Buffers and Salt Solutions are frequently used in tissue culture, cell isolation procedures, and molecular biology.For convenience, PromoCell offers ready-to-use Buffers and Salt Solutions. They are prepared from raw materials of the highest quality using tissue culture grade water.HEPES Buffered Saline Solution (30 mM).
Chem-Impex International, Inc. Bicine | 150-25-4 | MFCD00004295 | 100G
Bicine, 150-25-4, MFCD00004295, 100G
![Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 5 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659912.jpg-250.jpg)
Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 5 Kg
HEPES, Sodium Salt is a versatile biochemical reagent used to maintain a stable pH in biological and chemical applications. This 5kg package is ideal for large-scale laboratory use, providing ample quantities for high-volume buffer preparation. HEPES sodium salt is effective in the pH range of 6.8–8.2, making it suitable for a wide range of applications including cell culture, protein analysis, and enzymatic reactions. Its high buffering capacity and low toxicity to cells make it a reliable reagent for maintaining physiological pH during extended experiments. This bulk size ensures that laboratories can perform large-scale biochemical and biological studies with consistent results.
![Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-5000.0.JPG-250.jpg)
Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 5 Kilograms
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9